From the 7 March 2016 University of Toronto news release
Professor Milica Radisic and her team have created a new platform for growing realistic human heart and liver tissue outside the body. The technique could help drug companies discover and prevent negative side effects. (Photo: Caz Zyvatkauskas)
Researchers at U of T Engineering have developed a new way of growing realistic human tissues outside the body. Their “person-on-a-chip” technology, called AngioChip, is a powerful platform for discovering and testing new drugs, and could eventually be used to repair or replace damaged organs.
Professor Milica Radisic (IBBME, ChemE), graduate student Boyang Zhang and their collaborators are among those research groups around the world racing to find ways to grow human tissues in the lab, under conditions that mimic a real person’s body. They have developed unique methods for manufacturing small, intricate scaffolds for individual cells to grow on. These artificial environments produce cells and tissues that resemble the real thing more closely than those grown lying flat in a petri dish.
Left to right: Team members Miles Montgomery, Professor Milica Radisic, Boyang Zhang and Yimu Zhao (Photo: Geoff George)
The team’s recent creations have included BiowireTM — an innovative method of growing heart cells around a silk suture — as well as a scaffold for heart cells that snaps together like sheets of Velcro™. But AngioChip takes tissue engineering to a whole new level. “It’s a fully three-dimensional structure complete with internal blood vessels,” says Radisic. “It behaves just like vasculature, and around it there is a lattice for other cells to attach and grow.” The work — which is published todayin the journal Nature Materials — was produced collaboratively with researchers from across U of T, including Professor Michael Sefton (ChemE, IBBME), Professor Aaron Wheeler (Chemistry, IBBME) and their research teams, as well as researchers from Toronto General Hospital and University Health Network.
Zhang built the scaffold out of POMaC, a polymer that is both biodegradable and biocompatible. The scaffold is built out of a series of thin layers, stamped with a pattern of channels that are each about 50 to 100 micrometres wide. The layers, which resemble the computer microchips, are then stacked into a 3D structure of synthetic blood vessels. As each layer is added, UV light is used to cross-link the polymer and bond it to the layer below.
These tiny polymer scaffolds contain channels that are about 100 micrometres wide, about the same diameter as a human hair. When seeded with cells, the channels act as artificial blood vessels. By mimicking tissues in the human heart and other organs, these scaffolds provide a new way to test drugs for potentially dangerous side effects. (Image: Tyler Irving/Boyang Zhang/Kevin Soobrian)
When the structure is finished, it is bathed in a liquid containing living cells. The cells quickly attach to the inside and outside of the channels and begin growing just as they would in the human body.
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From the 12 December 2012 article at Science News Daily
Left: The iTube platform, which utilizes colorimetric assays and a smart phone-based digital reader. Right: A screen capture of the iTube App. (Credit: Image courtesy of University of California – Los Angeles)
Are you allergic to peanuts and worried there might be some in that cookie? Now you can find out using a rather unlikely source: your cell phone.
A team of researchers from the UCLA Henry Samueli School of Engineering and Applied Science has developed a lightweight device called the iTube, which attaches to a common cell phone to detect allergens in food samples. The iTube attachment uses the cell phone’s built-in camera, along with an accompanying smart-phone application that runs a test with the same high level of sensitivity a laboratory would….
To test for allergens, food samples are initially ground up and mixed in a test tube with hot water and an extraction solvent; this mixture is allowed to set for several minutes. Then, following a step-by-step procedure, the prepared sample is mixed with a series of other reactive testing liquids. The entire preparation takes roughly 20 minutes. When the sample is ready, it is measured optically for allergen concentration through the iTube platform, using the cell phone’s camera and a smart application running on the phone.
The kit digitally converts raw images from the cell-phone camera into concentration measurements detected in the food samples. And beyond just a “yes” or “no” answer as to whether allergens are present, the test can also quantify how much of an allergen is in a sample, in parts per million.
The iTube platform can test for a variety of allergens, including peanuts, almonds, eggs, gluten and hazelnuts, Ozcan said.
The UCLA team successfully tested the iTube using commercially available cookies, analyzing the samples to determine if they had any harmful amount of peanuts, a potential allergen. Their research was recently published online in the peer-reviewed journal Lab on a Chip and will be featured in a forthcoming print issue of the journal….